Rimi Sen was born as Subhamitra Sen in Kolkata, West Bengal. She completed her schooling from Bidya Bharati Girls' High School in 1998. She completed her graduation in Commerce from University of Calcutta. Rimi made her debut as a lead actress in the Telugu movie Nee Thodu Kavali. Her debut Hindi film, Vijay Galani's Hungama, released in 2003. It was a comedy film, in which she co-starred with Akshay Khanna, Aftab Shivdasani, and Paresh Rawal. She followed it with appearances in big budget movies like Dhoom, Kyon Ki, Garam Masala and Golmaal. She also did a cameo in the 2006 film Dhoom 2, and followed it with Johnny Gaddaar alongside newcomer actor Neil Nitin Mukesh.